SS-PCR (sequence specific primers-polymerase chain reaction) specific primers and kit for quickly identifying pumpkin fruit-fly

The invention relates to the field of quarantine pest detection, particularly an SS-PCR (sequence specific primers-polymerase chain reaction) specific primer pair and kit for quickly identifying pumpkin fruit-fly. The primer pair comprises a primer NF404: TGCCTTAGCGATATTCTGGCT and a primer NR610:AAC...

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Bibliographische Detailangaben
Hauptverfasser:	LIN YANGWU, CHEN SHAOPING, HUANG ZHEN, SHEN JIANGUO, LOU DINGFENG, GUO QIONGXIA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of quarantine pest detection, particularly an SS-PCR (sequence specific primers-polymerase chain reaction) specific primer pair and kit for quickly identifying pumpkin fruit-fly. The primer pair comprises a primer NF404: TGCCTTAGCGATATTCTGGCT and a primer NR610:AACTGTGTTCAGCAGGTGGT. The SS-PCR method for quickly identifying pumpkin fruit-fly is quick and simple, has the advantages of high specificity, high sensitivity and the like, can complete pumpkin fruit-fly identification within 8 hours, and can satisfy the requirements for seaport inspection/quarantine quick clearance.