Micro-carrier for cell culture as well as preparation method and application of micro-carrier
The invention relates to a micro-carrier for cell culture as well as a preparation method and application of the micro-carrier. The carrier comprises positive charge and a protein/peptide coating. The preparation method of the micro-carrier comprises the following steps: (1) carrying out chemical cr...
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| creator | SU ZHIGUO LI JUAN SUN LIJING ZHOU WEIQING WANG SHAOJIU MA GUANGHUI |
| description | The invention relates to a micro-carrier for cell culture as well as a preparation method and application of the micro-carrier. The carrier comprises positive charge and a protein/peptide coating. The preparation method of the micro-carrier comprises the following steps: (1) carrying out chemical crosslinking on matrix of the micro-carrier and positive charge modifier; and (2) crosslinking matrix microspheres with positive charge obtained from the step (1) and protein/peptide. The invention further provides application of the micro-carrier in cell culture. The micro-carrier is capable of limiting the thickness of the protein/peptide coating, so that the micro-carrier can fully play a role of the protein/peptide, does not completely shield the charge, has the advantage that positive charge is easily adhered to cells, and also has the functions of promoting the protein/peptide and regulating and controlling cell growth, so as to play dual roles of the charge and the protein/peptide and effectively regulate and control the amount; and the micro-carrier has obvious advantages in cell culture and tissue engineering. |
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The carrier comprises positive charge and a protein/peptide coating. The preparation method of the micro-carrier comprises the following steps: (1) carrying out chemical crosslinking on matrix of the micro-carrier and positive charge modifier; and (2) crosslinking matrix microspheres with positive charge obtained from the step (1) and protein/peptide. The invention further provides application of the micro-carrier in cell culture. The micro-carrier is capable of limiting the thickness of the protein/peptide coating, so that the micro-carrier can fully play a role of the protein/peptide, does not completely shield the charge, has the advantage that positive charge is easily adhered to cells, and also has the functions of promoting the protein/peptide and regulating and controlling cell growth, so as to play dual roles of the charge and the protein/peptide and effectively regulate and control the amount; and the micro-carrier has obvious advantages in cell culture and tissue engineering.</description><language>eng</language><subject>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS BASED THEREON ; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; GENERAL PROCESSES OF COMPOUNDING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC MACROMOLECULAR COMPOUNDS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; THEIR PREPARATION OR CHEMICAL WORKING-UP ; VINEGAR ; WINE ; WORKING-UP</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150610&DB=EPODOC&CC=CN&NR=104694454A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150610&DB=EPODOC&CC=CN&NR=104694454A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SU ZHIGUO</creatorcontrib><creatorcontrib>LI JUAN</creatorcontrib><creatorcontrib>SUN LIJING</creatorcontrib><creatorcontrib>ZHOU WEIQING</creatorcontrib><creatorcontrib>WANG SHAOJIU</creatorcontrib><creatorcontrib>MA GUANGHUI</creatorcontrib><title>Micro-carrier for cell culture as well as preparation method and application of micro-carrier</title><description>The invention relates to a micro-carrier for cell culture as well as a preparation method and application of the micro-carrier. 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The micro-carrier is capable of limiting the thickness of the protein/peptide coating, so that the micro-carrier can fully play a role of the protein/peptide, does not completely shield the charge, has the advantage that positive charge is easily adhered to cells, and also has the functions of promoting the protein/peptide and regulating and controlling cell growth, so as to play dual roles of the charge and the protein/peptide and effectively regulate and control the amount; and the micro-carrier has obvious advantages in cell culture and tissue engineering.</description><subject>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS BASED THEREON</subject><subject>COMPOSITIONS OF MACROMOLECULAR COMPOUNDS</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>GENERAL PROCESSES OF COMPOUNDING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC MACROMOLECULAR COMPOUNDS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>THEIR PREPARATION OR CHEMICAL WORKING-UP</subject><subject>VINEGAR</subject><subject>WINE</subject><subject>WORKING-UP</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2015</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZIj1zUwuytdNTiwqykwtUkjLL1JITs3JUUguzSkpLUpVSCxWKAfxgXRBUWpBYlFiSWZ-nkJuaklGfopCYh4QFxTkZCZDhPPTFHKRzeNhYE1LzClO5YXS3AyKbq4hzh66qQX58anFBYnJqXmpJfHOfoYGJmaWJiamJo7GxKgBANnPPBU</recordid><startdate>20150610</startdate><enddate>20150610</enddate><creator>SU ZHIGUO</creator><creator>LI JUAN</creator><creator>SUN LIJING</creator><creator>ZHOU WEIQING</creator><creator>WANG SHAOJIU</creator><creator>MA GUANGHUI</creator><scope>EVB</scope></search><sort><creationdate>20150610</creationdate><title>Micro-carrier for cell culture as well as preparation method and application of micro-carrier</title><author>SU ZHIGUO ; LI JUAN ; SUN LIJING ; ZHOU WEIQING ; WANG SHAOJIU ; MA GUANGHUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN104694454A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2015</creationdate><topic>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS BASED THEREON</topic><topic>COMPOSITIONS OF MACROMOLECULAR COMPOUNDS</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>GENERAL PROCESSES OF COMPOUNDING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC MACROMOLECULAR COMPOUNDS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>THEIR PREPARATION OR CHEMICAL WORKING-UP</topic><topic>VINEGAR</topic><topic>WINE</topic><topic>WORKING-UP</topic><toplevel>online_resources</toplevel><creatorcontrib>SU ZHIGUO</creatorcontrib><creatorcontrib>LI JUAN</creatorcontrib><creatorcontrib>SUN LIJING</creatorcontrib><creatorcontrib>ZHOU WEIQING</creatorcontrib><creatorcontrib>WANG SHAOJIU</creatorcontrib><creatorcontrib>MA GUANGHUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SU ZHIGUO</au><au>LI JUAN</au><au>SUN LIJING</au><au>ZHOU WEIQING</au><au>WANG SHAOJIU</au><au>MA GUANGHUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Micro-carrier for cell culture as well as preparation method and application of micro-carrier</title><date>2015-06-10</date><risdate>2015</risdate><abstract>The invention relates to a micro-carrier for cell culture as well as a preparation method and application of the micro-carrier. 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The micro-carrier is capable of limiting the thickness of the protein/peptide coating, so that the micro-carrier can fully play a role of the protein/peptide, does not completely shield the charge, has the advantage that positive charge is easily adhered to cells, and also has the functions of promoting the protein/peptide and regulating and controlling cell growth, so as to play dual roles of the charge and the protein/peptide and effectively regulate and control the amount; and the micro-carrier has obvious advantages in cell culture and tissue engineering.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OF MACROMOLECULAR COMPOUNDS COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY GENERAL PROCESSES OF COMPOUNDING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE WORKING-UP |
| title | Micro-carrier for cell culture as well as preparation method and application of micro-carrier |
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