Micro-carrier for cell culture as well as preparation method and application of micro-carrier

The invention relates to a micro-carrier for cell culture as well as a preparation method and application of the micro-carrier. The carrier comprises positive charge and a protein/peptide coating. The preparation method of the micro-carrier comprises the following steps: (1) carrying out chemical crosslinking on matrix of the micro-carrier and positive charge modifier; and (2) crosslinking matrix microspheres with positive charge obtained from the step (1) and protein/peptide. The invention further provides application of the micro-carrier in cell culture. The micro-carrier is capable of limiting the thickness of the protein/peptide coating, so that the micro-carrier can fully play a role of the protein/peptide, does not completely shield the charge, has the advantage that positive charge is easily adhered to cells, and also has the functions of promoting the protein/peptide and regulating and controlling cell growth, so as to play dual roles of the charge and the protein/peptide and effectively regulate and control the amount; and the micro-carrier has obvious advantages in cell culture and tissue engineering.