Bacteria culture medium and preparation method thereof

The invention discloses a bacteria culture medium and a preparation method thereof. A nutrient agar medium is taken as a basic culture medium, wherein a water-retaining agent and a coloring agent are added in the basic culture medium, the weight ratio of the nutrient agar, the water, the water-retaining agent to the coloring agent is (37-39):1000:(0.2-4): (0.02-0.09); and during the preparation process, each component is weighed, mixed and sterilized. By adopting the water-retaining agent in the obtained bacteria culture medium, the moisture of the culture medium can be kept and the accuracy of the measuring result influenced by the water loss and dryness can be avoided; by adopting the coloring agent, most bacterial colonies are dyed red or pink so that the detecting result can be conveniently observed; and simultaneously the preparation method is reasonable in design and simple and convenient in operation.