Recombinant vector and its preparation method and use

The invention provides a recombinant vector and its preparation method and use. The recombinant vector is constructed from a yeast secretory expression vector pPICZaA, contains a human-derived CTLA4 extracellular region gene for expression of a human-derived CTLA4 fusion protein, also contains a Kex...

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Bibliographische Detailangaben
Hauptverfasser:	WAN XIAOCHUN, RUAN QINGGUO, CHEN FENGLIAN, ZHAO QI, WANG PU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a recombinant vector and its preparation method and use. The recombinant vector is constructed from a yeast secretory expression vector pPICZaA, contains a human-derived CTLA4 extracellular region gene for expression of a human-derived CTLA4 fusion protein, also contains a Kex2 protease cleavage site, His label and c-myc label, can release the expressed CTLA4 fusion protein in a secretory protein form out of cells, realizes purification of the protein, and provides a simple method for CTLA4 protein industrialization.