Display substrate, manufacture method thereof, display panel and mask plate

The invention provides a display substrate. The display substrate comprises an anode layer, a cathode layer, and a luminous layer arranged between the anode layer and the cathode layer, wherein the anode layer comprises a plurality of anodes; the display substrate further comprises at least one auxiliary electrode; the auxiliary electrode and the anodes are formed at intervals in an insulated way; the auxiliary electrode is in contact with the cathode layer in a parallel connection way, so that the overall resistance formed after the auxiliary electrode is connected with the cathode layer in parallel is smaller than the resistance of the cathode layer. The invention further provides a display panel, a manufacture method of the display substrate, and a mask plate. The cathode resistance voltage drop (namely IR drop) of the display substrate is small, so that loss of electrical signals cathode layer in the cathode layer and the auxiliary electrode is small, and relatively higher image quality can be obtained.