Preparation method and detection method of rabies virus homogeneous fluorescent composite microsphere

The invention discloses a preparation method and detection method of a rabies virus homogenous fluorescent composite microsphere. Polystyrene nano microsphere is taken as the functionalized internal core to mark the fluorescent substance Eu3+, and at the same time a complexing agent BHHCT is used to mark recombinant rabies virus antigens so as to produce the rabies virus homogeneous fluorescent composite microsphere. The prepared rabies virus homogenous fluorescent composite microsphere is used to detect rabies virus antibody without dissociation or enhancement, moreover, the surface of the homogenous fluorescent composite microsphere is covered by a protective layer made of amino silane, and thus the external affection on the fluorescent signals is reduced, the fluorescence has a long life, so that the requirements of time-resolved fluoro-immune assay can be fulfilled. The provided detection method by using the rabies virus homogenous composite microsphere has the advantages of high sensitivity, low detection cost, and reduced operation steps and reaction time, the automatic operation can be achieved more easily, and the linear range of instrument reading is wider.