Isothermal amplification detection primer of Macadamia allergens, kit and method thereof
The invention discloses an isothermal amplification detection primer of Macadamia allergens, a kit and a method thereof. The method is based on a loop-mediated isothermal amplification technology, six specific primers are designed according to a specific target gene sequence AMP2 of macadimia nut, u...
Gespeichert in:
Hauptverfasser: | , , , , , , , , |
---|---|
Format: | Patent |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses an isothermal amplification detection primer of Macadamia allergens, a kit and a method thereof. The method is based on a loop-mediated isothermal amplification technology, six specific primers are designed according to a specific target gene sequence AMP2 of macadimia nut, under strand displacement activity-possessing Bst2.0WarmStarDNA polymerase effect, nucleic acid amplification is carried out for 45-60minutes at 63-65 DEG C, and the amplification efficiency can reach 109-1010 copy numbers. The method has the advantages of rapidity and high efficiency, simple operation, high specialty, high sensitivity and simple identification, and is suitable for on-site detection. |
---|