Isothermal amplification detection primer of Bertholletia excelsa allergens, kit and method thereof

The invention discloses an isothermal amplification detection primer of Bertholletia excelsa allergens, a kit and a method thereof. The method is based on a loop-mediated isothermal amplification technology, six specific primers are designed according to a coding gene sequence of Bertholletia excels...

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Bibliographische Detailangaben
Hauptverfasser:	LIU QING, GUAN LIJUN, LIU JIN, HE RIRONG, LI JIANJUN, XI JING, LI TING, LI ZHIYONG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an isothermal amplification detection primer of Bertholletia excelsa allergens, a kit and a method thereof. The method is based on a loop-mediated isothermal amplification technology, six specific primers are designed according to a coding gene sequence of Bertholletia excels 2S protein, under strand displacement activity-possessing Bst2.0WarmStarDNA polymerase effect, nucleic acid amplification is carried out for 45-60minutes at 63-65 DEG C, and the amplification efficiency can reach 109-1010 copy numbers. The method has the advantages of rapidity and high efficiency, simple operation, high specialty, high sensitivity and simple identification, and is suitable for on-site detection.