Method for producing hog cholera C-strain virus by culturing ST Cells in low serum

The invention discloses a method for producing a hog cholera C-strain virus by culturing ST Cells in low serum, and belongs to the technical field of veterinary medicine and biology. The method comprises the following steps: S1, preparing a cell subculture and culture solution; S2, domesticating cells to adapt to a low-serum culture medium; S3, domesticating cells to adapt to a low-serum culture environment; S4, breeding a cell virus strain; S5, breeding prepared venom. The method for producing the hog cholera C-strain virus by culturing ST Cells in low serum provided by the invention can remarkably reduce the production cost and can also improve the downstream purifying efficiency, and can quickly and stably expand production scale, so that the quality is easy to balance and stabilize.