Crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide

The invention discloses a crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide. The method is characterized by comprising the following steps: performing microwave dehydration of crateva unilocalaris Buch., wherein...

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Hauptverfasser: HUANG SHIJIE, RONG DEFU, LI XUELIAN, ZHENG JIANJUN
Format: Patent
Sprache:eng
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Zusammenfassung:The invention discloses a crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide. The method is characterized by comprising the following steps: performing microwave dehydration of crateva unilocalaris Buch., wherein the microwave power is 650W, and the dehydration time is 10min; milling the dehydrated crateva unilocalaris Buch. into dry crateva unilocalaris Buch. powder with the particle size of 40 meshes through a knife mill; placing the dry crateva unilocalaris Buch. powder in a reflux device, adding an ethanol solution with the volume concentration of 95%; performing reflux extraction for 3.6h at a constant temperature 97DEG C to obtain crude crateva unilocalaris Buch. polysaccharide; dissolving the crude crateva unilocalaris Buch. polysaccharide in water; purifying the crude crateva unilocalaris Buch. polysaccharide repeatedly by a Sevag method until the crude crateva unilocalaris Buch. polysaccharide has no characteristic absorption peak at 260nm and 280nm in an ultraviolet test; then performing washing and precipitation 3 times with acetone and ethyl acetate in sequence; and finally performing drying at 50DEG C to obtain crateva unilocalaris Buch. polysaccharide with the purity of 99.2%. The crateva unilocalaris Buch. polysaccharide can be applied to preparation of an antioxidant and has the advantages of high extraction efficiency, high product purity and high antioxidant performance.