Super-resolution fluorescence spectrum imaging microscope

The invention discloses a super-resolution fluorescence spectrum imaging microscope. The super-resolution fluorescence spectrum imaging microscope comprises a laser emitter, a dichroic mirror, a spectroscope, an image controller and a spectrograph, the laser emitter stimulates fluorophores of a samp...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: CHEN LONGCHAO, ZHENG WEI
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention discloses a super-resolution fluorescence spectrum imaging microscope. The super-resolution fluorescence spectrum imaging microscope comprises a laser emitter, a dichroic mirror, a spectroscope, an image controller and a spectrograph, the laser emitter stimulates fluorophores of a sample to emit fluorescence, the spectroscope divides the fluorescence into a first beam of light path and a second beam of light path, both the first beam of light path and second beam of light path have fluorescence signals of the fluorescence and spurious signals of exciting light, the first beam of light path is emitted to the image controller to gather the intensity information of the fluorescence, the second beam of light path is emitted to the spectrograph to analyze the spectrum information of the fluorescence and distinguish fluorescence signals from different fluorophores in the fluorescence according to the spectrum information, and the spectrum information gathered by the spectrograph is used for distinguishing the fluorescence signals of the fluorescence and spurious signals of the exciting light so as to distinguish the exciting light from the fluorescence signals through the spectrograph and improve the imaging effect of the super-resolution fluorescence spectrum imaging microscope.