Method for producing ring II type DBN-SX07 strain virus for pigs by seroculturing PK-15 cells

The invention discloses a method for producing ring II type DBN-SX07 strain virus for pigs by seroculturing PK-15 cells, and belongs to the technical field of biology of veterinaries. The method comprises the following steps: S1. performing passage and culture of cells; S2. domesticating the cells t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	HU LAIGEN, YUE FENGXIONG, XU HONGJUN, MOU HEPING, TIAN WEI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a method for producing ring II type DBN-SX07 strain virus for pigs by seroculturing PK-15 cells, and belongs to the technical field of biology of veterinaries. The method comprises the following steps: S1. performing passage and culture of cells; S2. domesticating the cells to enable the cells to be adapted for low seroculture bases; S3.domesticating the cells to enable the cells to be adapted for low seroculture environment; S4. propagation of cell virus seeds; S5. propagation of a prepared toxin liquid. The method for producing ring II type DBN-SX07 strain virus for pigs by seroculturing PK-15 cells, provided by the invention, can reduce the production cost obviously, besides, can improve the downstream purifying efficiency, and can rapidly and stably extend the production scale. Through the adoption of the method, balancing and stabilizing the quality is easy to realize.