Therapeutic HBV minicircle DNA vaccine and preparation method thereof

The invention provides a novel therapeutic HBV minicircle DNA vaccine. The vaccine comprises a minicircle DNA plasmid pMCS2.S for coding a HBV outer envelope protein preS2.S gene and a minicircle DNA plasmid pMCIIF for coding a hIL2-IFN gamma fusion protein gene. The plasmids pMCS2.S and pMCIIF do n...

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Bibliographische Detailangaben
Hauptverfasser:	WANG HONGMIN, CHEN GUANGMING, ZHANG HUANJING, RAO GUIRONG, YANG FUQIANG, MO GUOYU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a novel therapeutic HBV minicircle DNA vaccine. The vaccine comprises a minicircle DNA plasmid pMCS2.S for coding a HBV outer envelope protein preS2.S gene and a minicircle DNA plasmid pMCIIF for coding a hIL2-IFN gamma fusion protein gene. The plasmids pMCS2.S and pMCIIF do not contain a replication element and an antibiotic element of a prokaryotic plasmid, can be dissociated from host cell genome DNA, can stably and enduringly express an exogenous target gene, successfully realizes expression of an exogenous gene in COS-7 cells and induction of production of Th1 type immune response and specific killing response aiming at high-level IFN gamma of hepatitis b viruses in Balb/c mice.