Preparation method of microbiological leaching liquid of realgar

The invention discloses a preparation method of microbiological leaching liquid of realgar. Microbes are thiobacillus ferrooxidans. The preparation method of the microbiological leaching liquid of realgar comprises the following steps: screening strains, culturing acidophilic thiobacillus ferrooxidans and preparing the microbiological leaching liquid of realgar. According to the preparation method, the thiobacillus ferrooxidans are trained, so that the thiobacillus ferrooxidans have obvious effects of leaching mineral medicine realgar and can be used for degrading the realgar to a great extent; experimental results show that the concentration of As ions in the realgar which is leached by thiobacillus ferrooxidans after 30 days reaches 986.2 micrograms per micro-liter and is highly superior to that of As ions in powder-refining with water which is 82.7 micrograms per micro-liter; the thiobacillus ferrooxidans widely exist in acidic mine water and iron-containing or sulfur-containing acid environment and can be prepared by separating and extracting by virtue of simple experiments.