Serotyping PCR (polymerase chain reaction) method for haemophilus parasuis

The invention discloses a PCR (polymerase chain reaction) method for designing multiple pairs of primers for 15 serum types by comparing conserved genes on a capsule polysacharide locus of haemophilus parasuis and analyzing conserved gene difference among the serum types, screening a pair of primers...

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Bibliographische Detailangaben
Hauptverfasser:	JIA AIQING, WANG GUIPING, WANG YUEYUN, ZHOU RUYUE, AN XINYU, LIU QI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a PCR (polymerase chain reaction) method for designing multiple pairs of primers for 15 serum types by comparing conserved genes on a capsule polysacharide locus of haemophilus parasuis and analyzing conserved gene difference among the serum types, screening a pair of primers as serotyping PCR primers and establishing and identifying the 15 serum types of the haemophilus parasuis. The invention provides the serotyping PCR method for the haemophilus parasuis, which is high in accuracy, high in sensitivity and high in specificity.