Nanobacteria culture and morphological identification method

The invention discloses a nanobacteria culture and morphological identification method. The method comprises the following steps: performing sterile operation and recording primary urine as VB1, recording middle-section urine as VB2, EPS, and recording primary urine as VB3 after massage; treating an EPS specimen; treating a calculus specimen; culturing the specimen; performing indirect immunofluorescence staining and fluorescence microscope observation; performing transmission electron microscope scanning; performing ultrathin section observation; performing scanning electron microscope observation; performing Dahl McGec-Russel Alizarin red staining, thus obtaining the result determination standard. The EPS specimen and the calculus specimen are treated according to the standard procedure so as to be subjected to bacteria culture and identification, pathogenic bacteria are collected for researching the possible pathogenesis relationship between nanobacteria and type III prostatitis and prostatic calculus, the method is well used for guiding the clinical work, the pain of the mind and body and economical burdens of patients can be alleviated, and more medical resources are saved.