Method for producing trichosanthes tissue culture seedlings

Method for producing trichosanthes tissue culture seedlings

The invention discloses a method for producing trichosanthes tissue culture seedlings. The method comprises the following steps: (1) collecting single-node stems with axillary buds from strong trichosanthes plants, and performing conventional disinfection and sterilization; and (2) cutting the stems...

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Hauptverfasser: XU HONGYUAN, LIANG QIONGYUE, XU HAOXIANG, ZHOU FENGJUE, MO SHUMEI, GONG YINHUA
Format: Patent
Sprache:eng
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AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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creator XU HONGYUAN
LIANG QIONGYUE
XU HAOXIANG
ZHOU FENGJUE
MO SHUMEI
GONG YINHUA
description The invention discloses a method for producing trichosanthes tissue culture seedlings. The method comprises the following steps: (1) collecting single-node stems with axillary buds from strong trichosanthes plants, and performing conventional disinfection and sterilization; and (2) cutting the stems into an MS improved solid medium added with LFS for performing trichosanthes tissue culture seedling production, wherein the formula of the MS improved solid medium is 0.1-0.5mg.L(MS+LFS). The MS improved solid medium can induce explants to directly grow to the trichosanthes tissue culture seedlings with complete into roots and buds. The method disclosed by the invention has the advantages of simple production procedure, high tissue culture seedling quality and low cost.
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The method comprises the following steps: (1) collecting single-node stems with axillary buds from strong trichosanthes plants, and performing conventional disinfection and sterilization; and (2) cutting the stems into an MS improved solid medium added with LFS for performing trichosanthes tissue culture seedling production, wherein the formula of the MS improved solid medium is 0.1-0.5mg.L&lt;-1&gt;(MS+LFS). The MS improved solid medium can induce explants to directly grow to the trichosanthes tissue culture seedlings with complete into roots and buds. 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subjects AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
title Method for producing trichosanthes tissue culture seedlings
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