Method for producing trichosanthes tissue culture seedlings

The invention discloses a method for producing trichosanthes tissue culture seedlings. The method comprises the following steps: (1) collecting single-node stems with axillary buds from strong trichosanthes plants, and performing conventional disinfection and sterilization; and (2) cutting the stems...

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Hauptverfasser:	XU HONGYUAN, LIANG QIONGYUE, XU HAOXIANG, ZHOU FENGJUE, MO SHUMEI, GONG YINHUA
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING
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Beschreibung
Zusammenfassung:	The invention discloses a method for producing trichosanthes tissue culture seedlings. The method comprises the following steps: (1) collecting single-node stems with axillary buds from strong trichosanthes plants, and performing conventional disinfection and sterilization; and (2) cutting the stems into an MS improved solid medium added with LFS for performing trichosanthes tissue culture seedling production, wherein the formula of the MS improved solid medium is 0.1-0.5mg.L(MS+LFS). The MS improved solid medium can induce explants to directly grow to the trichosanthes tissue culture seedlings with complete into roots and buds. The method disclosed by the invention has the advantages of simple production procedure, high tissue culture seedling quality and low cost.