QAMS for detecting content of organic acids in sowthistle-leaf ixeris seedling injection
The invention relates to the field of analysis of traditional Chinese medicine components, and relates to QAMS for detecting the content of organic acids in sowthistle-leaf ixeris seedling injection. The QAMS disclosed by the invention comprises the following steps: (1), setting chromatographic cond...
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Zusammenfassung: | The invention relates to the field of analysis of traditional Chinese medicine components, and relates to QAMS for detecting the content of organic acids in sowthistle-leaf ixeris seedling injection. The QAMS disclosed by the invention comprises the following steps: (1), setting chromatographic conditions; (2), preparing a reference solution; (3), preparing a test solution; and (4), calculating correction factors. Relative correction factors of chicoric acid, mono-caffeoyl tartaric acid, chlorogenic acid and caffeic acid are calculated by taking chicoric acid as the internal reference compound, and used as constants for detecting the content; correction factors are calculated by taking effective active components in the sowthistle-leaf ixeris seedling injection and high-content chicoric acid as internal reference compounds; the content of mono-caffeoyl tartaric acid, chlorogenic acid and caffeic acid in the sowthistle-leaf ixeris seedling injection are calculated; the content of the four organic acids in the injection can be detected simultaneously; therefore, the quality of the sowthistle-leaf ixeris seedling injection in industrial production can be monitored scientifically, sensitively and rapidly; and simultaneously, the problem that the quality of the sowthistle-leaf ixeris seedling injection cannot be controlled objectively and reasonably because of shortage of reference substances can be solved. |
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