Method for purifying edible fungus polysaccharide by using enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology

The invention relates to a method for purifying edible fungus polysaccharide by using an enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology. The method comprises the following steps: adding enzyme into crushed edible fungi, mixing with water, stirring, and performing enzymolysis for a certain period of time; performing microwave radiation for a period of time, then cooling and performing suction filtration to obtain a polysaccharide extract; concentrating the extract, then adding chitosan to perform flocculation, and filtering to remove deposition after flocculation is finished, thus obtaining a purified polysaccharide solution; and drying to obtain relatively pure edible fungus polysaccharide which is tested to have good vitro antioxidation activity. According to the method provided by the invention, an enzymolysis technology is adopted to perform a certain degree of hydrolysis destruction on cell walls and intercellular substances of edible fungi at first, and then a 'body heating' characteristic of microwave is adopted to promote the dissolution of active ingredients. The method is efficient and energy-saving and can reduce the solvent consumption; and chitosan serving as a natural flocculating agent can well remove protein high polymer impurities, is good in impurity removal effect and low in cost, and cannot influence the product quality.