Construction methods and application of fusion protein and vector thereof

The invention discloses construction methods and application of a fusion protein and a vector thereof and aims to provide a method for constructing a fusion protein scientifically and effectively by taking TALEN (transcription activator-like effector nuclease) as a cell strain. The method is technic...

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Hauptverfasser:	HUANG LONG, HUO YIRAN, LI HONGMEI, TAN QIHONG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses construction methods and application of a fusion protein and a vector thereof and aims to provide a method for constructing a fusion protein scientifically and effectively by taking TALEN (transcription activator-like effector nuclease) as a cell strain. The method is technically characterized in that the fusion protein has a structure of EGFP-_mODC_NLS, and an expression vector of the fusion protein comprises the fusion protein with the structure of EGFP-_mODC_NLS and an NLS cutting TALEN plasmid pair of the fusion protein. The fusion protein is used for the NLS cutting TALEN plasmid pair and a prokaryotic expression protein with the structure of EGFP_mODC_NLS_TALEN_R and a protein with the structure of EGFP_mODC_NLS_TALEN_R, and the fusion protein can be used for verifying the transfection efficiency of the TALEN plasmid pair and the activity of the TALEN plasmid pair in cells. The invention belongs to the field of biotechnology.