Preparation method of human dendritic cell tumour vaccine

The invention provides a preparation method of a human dendritic cell tumour vaccine and belongs to the technical field of cell engineering. The human dendritic cell tumour vaccine is characterized in that a DC maturity acceleration agent is added in a process that the dendritic cell is matured from being unmatured, and the DC maturity acceleration agent comprises OK-432 and a gp100 combined DC maturity acceleration agent. By adopting the human dendritic cell tumour vaccine, cancer patients can be treated or high risk of people suffering from cancer can be avoided. The human dendritic cell tumour vaccine prepared by adopting the preparation method provided by the invention has the advantages that DC purity and maturity of the human dendritic cell tumour vaccine are greatly improved, fusion of DC and a tumour antigen is promoted, and treatment of cancer can be facilitated; meanwhile, a preparation technology is simple, and cost is low.