Online determination method suitable for zearalenone and metabolites of zearalenone

The invention discloses an online enrichment and separation method suitable for zearalenone and metabolites of the zearalenone. The method comprises the following steps: by adopting a capillary electrochromatography technology, bonding a silica capillary packed column with octadecyl, or taking a polymer monolithic column formed by vinylbenzyl chloride as a functional monomer and cage octa(vinylsilsesquioxane) as a crosslinking agent as an organic-silica gel hybrid capillary monolithic column, and simultaneously exerting a negative charge high-voltage electric field and auxiliary pressure of a liquid-phase pump on the hybrid polymer monolithic column; and achieving on-column concentration and multi-component continuous separation of the zearalenone and the metabolites of the zearalenone by using dual effects of high-voltage electric field drive and capillary micro-flow liquid-phase distribution and separation, so as to achieve quantitative analysis of the zearalenone and the metabolites of the zearalenone. According to the online enrichment and separation method, multiple effects of online enrichment of the capillary column, high-voltage electric field drive and capillary micro-flow liquid-phase separation are comprehensively utilized; offline treatment on a to-be-detected component is not required; and the online enrichment and separation method is continuous in flow, and high in sensitivity, and can be applied to trace analysis of the zearalenone and the metabolites of the zearalenone.