Fluorescence RT-PCR primer, probe and kit for detecting Schmallenberg viruses

The invention discloses a fluorescence RT-PCR primer, a probe and a kit for detecting Schmallenberg viruses. The sequences of the primer and the probe are shown as SEQ ID NO. 1-6. The kit comprises the primer, the probe, reverse transcriptase, DNA polymerase, an RNA extraction reagent, a fluorescenc...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN XUAN, LUO BAOZHENG, HUANG HAICHAO, TAO MIN, XU HAINIE, LIAO XIUYUN, SHA CAIHUA, YANG SU, BO QINGRU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a fluorescence RT-PCR primer, a probe and a kit for detecting Schmallenberg viruses. The sequences of the primer and the probe are shown as SEQ ID NO. 1-6. The kit comprises the primer, the probe, reverse transcriptase, DNA polymerase, an RNA extraction reagent, a fluorescence PCR reaction reagent, a Schmallenberg virus positive control and a Schmallenberg virus negative control. The Schmallenberg virus fluorescence RT-PCR kit and a detection method provided by the invention are specific, sensitive and quick, have good repeatability and low costs, and the detection method is a favorable method for quickly detecting Schmallenberg viruses.