Dideoxyribonucleoside modified primer, method, reaction system and application thereof to mutation detection

The invention relates to a dideoxyribonucleoside modified primer, a method, a reaction system and application thereof to mutation detection. Through combination of a 3' terminal blocked primer and 3'-5' exonuclease correction function of a high fidelity DNA polymerase, the invention o...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ZHANG CHIYU, FAN LUJUAN, LAN KE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention relates to a dideoxyribonucleoside modified primer, a method, a reaction system and application thereof to mutation detection. Through combination of a 3' terminal blocked primer and 3'-5' exonuclease correction function of a high fidelity DNA polymerase, the invention optimizes a detection method with simple and quick operation, high sensitivity and high accuracy, and the method can be widely used in fields related to point mutation, single nucleotide polymorphism, insertion and deletion mutation.