Co-culture method of DC cell and CIK cell

The invention relates to a co-culture method of a DC cell and a CIK cell and belongs to the technical field of cell culture. The co-culture method comprises the following steps: 1) extracting a single nucleus cell; 2) culturing the CIK cell; 3) culturing the DC cell; 4) carrying out mixed culturing...

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Bibliographische Detailangaben
Hauptverfasser:	MENG YANFEN, YAN JINGWU, DU CHENGDE, CHENG SIBO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a co-culture method of a DC cell and a CIK cell and belongs to the technical field of cell culture. The co-culture method comprises the following steps: 1) extracting a single nucleus cell; 2) culturing the CIK cell; 3) culturing the DC cell; 4) carrying out mixed culturing on the DC cell and the CIK cell; and 5) carrying out multiplication culture. According to the co-culture method disclosed by the invention, after the DC cell is stimulated to be mature and co-cultured with the CIK cell, a factor GM-CSF is added according to the concentration and replenished after culture for 12h, so that the activity of the DC cell is kept, the survival time of the DC cell in a CIK culture system is prolonged, and the activity of the DC cell is kept for a long time.