Separation and detection method for anticoagulant drugs

The invention belongs to the field of analytical chemistry and particularly relates to a method of separating and testing (S)-Rivaroxaban and enantiomers thereof by liquid chromatography. The method for separating and testing (S)-Rivaroxaban and enantiomers (impurities) thereof by liquid chromatography is characterized in that a polysaccharide derivative is used as a chiral chromatographic column of filler and a mixed solution of low alcohol or low alkane or low alcohol is used as a moving phase. According to the separation and detection method provided by the invention, (S)-Rivaroxaban can be effectively separated from enantiomers thereof, wherein the separation degree reaches over 1.5 and complete baseline separation is achieved, so that the quality of (S)-Rivaroxaban can be accurately and effectively controlled. The separation method provided by the invention can be used for separating and detecting the (S)-Rivaroxaban and enantiomers thereof within 80 minutes. The method provided by the invention can be used for simply, quickly and accurately separating and detecting (S)-Rivaroxaban and optical isomers thereof.