Saccharomyces cerevisiae module co-transformation combined screening method

The invention discloses a saccharomyces cerevisiae module co-transformation combined screening method which comprises the following steps: (1) respectively selecting an element from a to-be-screened promoter element library, a to-be-screened genetic element library and a to-be-screened terminator element library each time to be assembled in plasmids with different selection markers so as to obtain modules, wherein the selection markers selected from the same genetic elements are the same, and a module library is composed of the modules; (2) forming a permutation and combination design on the modules in the module library; (3) transferring the modules which correspond to the design into saccharomyces cerevisiae base tray bacteria by utilizing a saccharomyces cerevisiae plasmid co-transformation technology; (4) culturing the transformed bacteria on a selection defect type culture medium, thereby obtaining saccharomyces cerevisiae transformants in an accurate permutation and combination mode; and (5) screening saccharomyces cerevisiae transformants in a high-efficiency module combination according to specific characteristics of target traits. According to the method disclosed by the invention, the workload is greatly reduced, and the flexibility of the constructed system is improved. The obtained combination does not need to be sequenced and therefore the cost is reduced.