Method for preparing aflatoxin B1 aptamer affinity column

The invention discloses a method for preparing an aflatoxin B1 aptamer affinity column and belongs to the field of affinity chromatography and mycotoxin analysis. The method comprises the following steps: adopting epoxy activated agarose microspheres FF as a solid phase carrier; after the solid phas...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LIAO QIEGEN, LI WEIHONG, HU LIFANG, LUO LINGUANG
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS SEPARATION TESTING THEIR RELEVANT APPARATUS TRANSPORTING
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a method for preparing an aflatoxin B1 aptamer affinity column and belongs to the field of affinity chromatography and mycotoxin analysis. The method comprises the following steps: adopting epoxy activated agarose microspheres FF as a solid phase carrier; after the solid phase carrier is coupled with an amino-modified aflatoxin B1 aptamer, closing extra active sites in the carrier, and feeding into micro columns. The prepared aptamer affinity column can be specially combined with aflatoxin B1, the adding standard recovery is 70-110, and the aptamer affinity column can be repeatedly used for at least five times.