Preparation method and use of hemp seed reference extract product

preparation method of a hemp seed reference extract product comprises the following steps: 1, carrying out ether degreasing: taking 200g of hemp seed medicinal material powder, sieving by a No.2 sieve, adding 1500ml of ether, carrying out water bath heating refluxing for 1h at a water bath temperature of 50DEG C, carrying out reduced pressure filtration, adding 500ml to medicinal residues, washing, and removing the obtained ether liquid; 2, extracting with methanol to obtain an extract: adding 1500ml to the obtained medicinal residues, carrying out water bath heating refluxing at 70DEG C for 1h for extraction, carrying out reduced pressure filtration, and evaporating to remove methanol to obtain an extract, and weighing; and 3, carrying out silicon gel mixing and reduced pressure drying: re-dissolving the extract obtained in step 2 in 100ml of methanol, adding column chromatography silica gel having a same amount with the extract, uniformly mixing, carrying out reduced pressure evaporation to remove methanol, and sieving the obtained substance by a No.9 sieve in order to obtain the hemp seed reference extract product. The invention also provides a use of the hemp seed reference extract product. The hemp seed reference extract product substitutes a hemp seed reference medicinal material to be used as a reference for thin layer chromatogram discrimination.