High-copy pTerm plasmid as well as construction method and application thereof

High-copy pTerm plasmid as well as construction method and application thereof

The invention relates to a high-copy pTerm-series plasmid as well as a construction method and application thereof. The pTerm-series plasmid comprises a colE1 replicon gene, a procaryotic transcription terminator and an antibiotic resistance gene. The high-copy pTerm-series plasmid disclosed by the...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: SUN ZHONGPING, XUE GAOXU, LIAO GUOJUAN, FENG AIHUA
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to a high-copy pTerm-series plasmid as well as a construction method and application thereof. The pTerm-series plasmid comprises a colE1 replicon gene, a procaryotic transcription terminator and an antibiotic resistance gene. The high-copy pTerm-series plasmid disclosed by the invention has the characteristics of high copy number, high capacity, high stability and the like, can be applied in the field of genetic engineering, such as cloning, screening and sequencing of complex-structure genes including repetitive sequences, instable genes and long-segment genes, constructing a genome and the like and has an important effect on high-efficiency and high-quality synthesis of genes.