Fusion protein for treating HPV16 and HPV18 viruses and preparation method of fusion protein
The invention discloses a fusion protein for treating HPV16 and HPV18 viruses and a preparation method of the fusion protein, and aims at providing the fusion protein which is simple in preparation method and is capable of effectively treating HPV16 and HPV18 viruses. According to the technical main...
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creator | WEN HUAJIE HUANG LONG TAN QIHONG |
description | The invention discloses a fusion protein for treating HPV16 and HPV18 viruses and a preparation method of the fusion protein, and aims at providing the fusion protein which is simple in preparation method and is capable of effectively treating HPV16 and HPV18 viruses. According to the technical main points, a gene sequence of the fusion protein TALEs-ScPvuII-HPV18 is as shown in SEQ ID NO:1; the gene sequence of the fusion protein TALEs-ScPvuII-HPV161-3 is as shown in SEQ ID NO:2-4. The preparation method comprises the following steps: 1) screening target sites; 2) building PET16b-TALEs-ScPvuII-HPV18 and PET16b-TALEs-ScPvuII-HPV161-3 prokaryotic expression vectors; and 3) expressing and purifying TALEs-ScPvuII-HPV18 and TALEs-ScPvuII-HPV161-3. |
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According to the technical main points, a gene sequence of the fusion protein TALEs-ScPvuII-HPV18 is as shown in SEQ ID NO:1; the gene sequence of the fusion protein TALEs-ScPvuII-HPV161-3 is as shown in SEQ ID NO:2-4. The preparation method comprises the following steps: 1) screening target sites; 2) building PET16b-TALEs-ScPvuII-HPV18 and PET16b-TALEs-ScPvuII-HPV161-3 prokaryotic expression vectors; and 3) expressing and purifying TALEs-ScPvuII-HPV18 and TALEs-ScPvuII-HPV161-3.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150304&DB=EPODOC&CC=CN&NR=104387475A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25547,76298</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150304&DB=EPODOC&CC=CN&NR=104387475A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WEN HUAJIE</creatorcontrib><creatorcontrib>HUANG LONG</creatorcontrib><creatorcontrib>TAN QIHONG</creatorcontrib><title>Fusion protein for treating HPV16 and HPV18 viruses and preparation method of fusion protein</title><description>The invention discloses a fusion protein for treating HPV16 and HPV18 viruses and a preparation method of the fusion protein, and aims at providing the fusion protein which is simple in preparation method and is capable of effectively treating HPV16 and HPV18 viruses. According to the technical main points, a gene sequence of the fusion protein TALEs-ScPvuII-HPV18 is as shown in SEQ ID NO:1; the gene sequence of the fusion protein TALEs-ScPvuII-HPV161-3 is as shown in SEQ ID NO:2-4. 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According to the technical main points, a gene sequence of the fusion protein TALEs-ScPvuII-HPV18 is as shown in SEQ ID NO:1; the gene sequence of the fusion protein TALEs-ScPvuII-HPV161-3 is as shown in SEQ ID NO:2-4. The preparation method comprises the following steps: 1) screening target sites; 2) building PET16b-TALEs-ScPvuII-HPV18 and PET16b-TALEs-ScPvuII-HPV161-3 prokaryotic expression vectors; and 3) expressing and purifying TALEs-ScPvuII-HPV18 and TALEs-ScPvuII-HPV161-3.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
title | Fusion protein for treating HPV16 and HPV18 viruses and preparation method of fusion protein |
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