Tissue culture rapid propagation method of eucalyptus urophylla DH32-28 variety

The invention discloses a tissue culture rapid propagation method of eucalyptus urophylla DH32-28 variety. The method comprises the steps of establishing an explant, sterilizing the explant, carrying out primary culture, carrying out subculture, carrying out rooting culture, exercising seedlings, tr...

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Hauptverfasser: SHEN YUN, WU MANFEN, DENG DONGLI, WU BING, TANG ZAISHENG, LIANG XIULI, FU MIAO, CHEN YUNCHUN, PANG ZHENWU, WEI BINGJIAN, SU YONG, GONG WEIXIN, CHEN DEWEN, LYU HUALI, HUANG QUANDONG, FENG RONGDI
Format: Patent
Sprache:eng
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Zusammenfassung:The invention discloses a tissue culture rapid propagation method of eucalyptus urophylla DH32-28 variety. The method comprises the steps of establishing an explant, sterilizing the explant, carrying out primary culture, carrying out subculture, carrying out rooting culture, exercising seedlings, transplanting and the like, wherein the special culture medium composition is selected aiming at the primary culture, the subculture and the rooting culture; after the reasonable measure of exercising seedlings is adopted, the eucalyptus urophylla is rapid in growth and high in survival rate after being transplanted, so that the aims of high propagation coefficient and good propagation effect can be achieved. Furthermore, the production method is low in production cost, and the obtained tissue culture seedlings are consistent in genetic characters, so that the method is an effective way for eucalyptus tissue culture rapid propagation and is wide in application scope.