Preparation of bordetella bronchiseptica selective medium

The invention relates to preparation of a bordetella bronchiseptica selective medium. The preparation comprises the following steps: taking 120g of NaCl, 20g of peptones, 18g of agar powder, 10g of glucose, 10g of lactose, 1.5g of No.3 cholate, 3ml of a 1% neutral red aqueous solution and 100ml of neonatal bovine serum; adding distilled water to a fixed volume of 1000ml; after dissolving and uniformly mixing, autoclaving for 15 minutes at 115 DEG C; cooling to 55 DEG C; adding a proper amount of a 1% furazolidone dimethylformamide solution, ampicillin, actidione and spectinomycin, taking 20ml of the solution, uniformly mixing and pouring into a plate with the diameter of 90mm; drying, collecting cotton swabs of naval cavities of pigs with atrophic rhinitis clinical symptoms; placing the cotton swabs into 2ml of a sterile PBS (phosphate buffer solution) solution; after fully and uniformly mixing, coating 50 microlitres on the selective medium; culturing for 48 hours at 37 DEG C; and picking up suspicious colonies and identifying, wherein the separation rate of the selective medium is remarkably higher than that of existing separating mediums.