Multi-mode non-linear optical microscopy imaging method and device

The invention relates to a multi-mode non-linear optical microscopy imaging method and device. The multi-mode non-linear optical microscopy imaging device mainly comprises a laser system, an optical scanning microscope and a non-linear optical signal detecting and acquiring system. The multi-mode non-linear optical microscopy imaging device can work in a single-laser-beam mode and a dual-laser-beam mode and can realize multi-mode non-linear optical microscopy imaging such as two-photon excited Fluorescence (TPEF) imaging, multiphoton high-order harmonic (such as second harmonic generation SHG, and third harmonic generation THG) scattering imaging, coherent Raman scattering (such as anti-Stokes CARS) microscopy imaging) on isolated biological tissues and living cells, so that various non-linear specific optical signals of biological tissue samples can be obtained in situ, so that the important basis is provided for the optical diagnosis and deep analysis of the samples. Besides, a reflection measurement manner disclosed by the invention can be further directly applied to the acquiring of various non-linear specific optical signals of live animals and the microscopy imaging.