Method for producing unsaturated fatty acid from gene engineering Escherichia coli

The invention discloses a method for producing unsaturated fatty acid from gene engineering Escherichia coli. Recombinant engineering Escherichia coli capable of co-expressing 3-hydroxyacyl ACP anhydrase gene fabA and 3-ketoacyl ACP synthetase gene fabB is fermented in a glucose-containing fermentat...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG QIUHAO, RAO BING, WEN YUQI, RONG HUI, OUYANG LI, CHEN YUDA, XU CHUNHUI, ZHU QIZHANG, QIN ZHEN, ZHOU YU, CHEN XI, ZHU YI, XU YILIN, ZHU RUICHI, YANG ZHANCHAO, ZHAO YILING
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for producing unsaturated fatty acid from gene engineering Escherichia coli. Recombinant engineering Escherichia coli capable of co-expressing 3-hydroxyacyl ACP anhydrase gene fabA and 3-ketoacyl ACP synthetase gene fabB is fermented in a glucose-containing fermentation culture medium to convert the recombinant engineering Escherichia coli into glucose, thereby obtaining the unsaturated fatty acid. The production technique has the advantages of mild conditions, no need of expensive catalyst and high product specificity, avoids harsh reaction conditions and prevents the byproducts from generation in the traditional chemical synthesis process, and is a green new technique for synthesizing unsaturated fatty acid.