Construction method for standard characteristic spectrum of hericium erinaceus mycelium, and quality detection method for hericium erinaceus mycelium based on standard characteristic spectrum
The invention discloses a construction method for a standard characteristic spectrum of hericium erinaceus mycelium, and a quality detection method for hericium erinaceus mycelium based on the standard characteristic spectrum. The method comprises the steps of (1) preparing a reference solution; (2)...
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Zusammenfassung: | The invention discloses a construction method for a standard characteristic spectrum of hericium erinaceus mycelium, and a quality detection method for hericium erinaceus mycelium based on the standard characteristic spectrum. The method comprises the steps of (1) preparing a reference solution; (2) preparing a test solution; (3) setting spectrum conditions: filler of a chromatography column is octadecylsilane chemically bonded silica, gradient elution is adopted, a mobile phase is a gradient elution solution composed of methanol and a 1% glacial acetic acid solution (by mass), a column temperature is 30-40 DEG C, a flow rate is 0.8-1.2 ml/min and a detection wavelength is 250-260 nm; and (4) determining the characteristic spectrum by high performance liquid chromatography. |
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