Method applied to chromosome production of single tobacco plant by tender ovary

The invention discloses a method applied to chromosome production of a single tobacco plant by tender ovary. The method comprises the following steps: (1) drawing materials; (2) preprocessing and fixing; and (3) subsequently preparing tobacco chromosome by methods of directly smearing, carrying out enzymolysis and then sheeting and smearing. According to the method, the tobacco tender ovary as the material is used for chromosome production; as the tobacco is high in quantity of flowers and long in flower period, a large quantity of materials can be selected; a large quantity of split phases are prepared; part of the split phases has good chromosome morphology and is applied to subsequent test analysis processes such as karyotype analysis and genomic in-situ hybridization. By virtue of the method, the defects of tiny and few materials during chromosome production of tobacco root tip can be overcome; and the method is especially suitable for the chromosome production of the single tobacco plant by the tender ovary.