Method for extracting SOD, CAT, NADH oxidase and ATP in white-rot fungi composite adsorbent simultaneously

Method for extracting SOD, CAT, NADH oxidase and ATP in white-rot fungi composite adsorbent simultaneously

The invention discloses a method for extracting SOD, CAT, NADH oxidase and ATP in a white-rot fungi composite adsorbent simultaneously. The method for extracting SOD, CAT, NADH oxidase and ATP in the white-rot fungi composite adsorbent simultaneously comprises the following steps: adding the white-r...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: GUO ZHI, HUANG ZHENZHEN, YAN MING, ZENG GUANGMING, CHEN ANWEI, ZUO YA'NAN, CHEN GUIQIU, TAN QIONG
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses a method for extracting SOD, CAT, NADH oxidase and ATP in a white-rot fungi composite adsorbent simultaneously. The method for extracting SOD, CAT, NADH oxidase and ATP in the white-rot fungi composite adsorbent simultaneously comprises the following steps: adding the white-rot fungi composite adsorbent into a CaCl2 solution to prepare small balls with white-rot fungi being embedded, and carrying out constant temperature shaking culture on the small balls with the white-rot fungi being embedded to obtain a culture solution; adding Cd into the culture solution for carrying out stress culture, then taking out the small balls with the white-rot fungi being embedded, and carrying out liquid nitrogen grinding to obtain a grinding sample; and adding phosphate buffer into the grinding sample, carrying out ultrasonic treatment in an ice bath, centrifuging to obtain a compound of SOD, CAT, NADH oxidase and ATP. The method for extracting SOD, CAT, NADH oxidase and ATP in the white-rot fungi composite adsorbent simultaneously has the advantages that heavy metal stress is adopted for improving enzyme activity of SOD, CAT and NADH oxidase and increasing ATP content, a cell breakage effect is good and extraction efficiency is high.