Nitrile degradation biofilm formed genetically engineered bacterium and application of genetically engineered bacterium in nitrile-containing wastewater treatment

The invention provides a nitrile degradation biofilm formed genetically engineered bacterium and application of the genetically engineered bacterium in nitrile-containing wastewater treatment, and belongs to the bioengineering technology. The genetically engineered bacterium Bacillus subtilis N4-pHT01-Nitr with the preservation number of CGMCC No.9484 is preserved in the China General Microbiological Culture Collection Center on July 25, 2014. The bacterium is prepared by inserting a nitrilase gene in Rhodococcus rhodochrous BX2 into Bacillus subtilis N4 for expressing by means of gene engineering. The bacterium is capable of generating a biofilm and degrading acetonitrile, the OD570nm value of biofilm formation capacity is 1.45 when the bacterium is culture in synthetic wastewater at 37 DEG C and 60r/min for 48 hours; and the residual concentration of acetonitrile is 23.56mg/L after the bacterium is oscillated and cultured in 800mg/L acetonitrile-containing synthetic wastewater at 37 DEG C and 160rpm for 48 hours. The bacterium has strong acetonitrile impact resistance, acetonitrile in synthetic wastewater is reduced from 800mg/L to 0.32mg/L when the wastewater is output, and the COD value of the synthetic wastewater is reduced from 487mg/L to 16.39mg/L when the wastewater is output. The bacterium provides a novel method to nitrile-containing wastewater treatment by utilizing a biofilm method.