Tissue culture and rapid propagation method of eucalyptus urophylla DH33 to DH27 varieties

The invention discloses a tissue culture and rapid propagation method of eucalyptus urophylla DH33 to DH27 varieties. The method comprises the following steps: establishing an explant, sterilizing the explant, carrying out primary culture, carrying out secondary culture, carrying out rooting culture, exercising seedlings, transplanting and the like. A special culture medium is selected for the primary culture, the secondary culture and the rooting culture, the eucalyptus urophylla can grow fast and is high in survival rate due to the reasonable seedling exercising means, and the purposes of high propagation coefficient and good propagation effect can be achieved. Meanwhile, the method is low in production cost, the genetic traits of the obtained tissue-culture seedlings are consistent, the method is an effective way for the tissue culture and rapid propagation of the eucalyptus, and the application range is wide.