Pinellia ternate medicinal material detection method
The invention relates to a medicinal material detection method and concretely relates to a method for detecting alkaloid content and pesticide residue content of a pinellia ternate medicinal material. The method comprises alkaloid content determination: by chloroform, extracting a sample to be detec...
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Format: | Patent |
Sprache: | eng |
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Zusammenfassung: | The invention relates to a medicinal material detection method and concretely relates to a method for detecting alkaloid content and pesticide residue content of a pinellia ternate medicinal material. The method comprises alkaloid content determination: by chloroform, extracting a sample to be detected so that the extract is obtained and is used as a sample solution, preparing a contrast solution from ephedrine hydrochloride, determining a light absorption value by ultraviolet analysis and carrying out comparison calculation, and pesticide residue content determination: preparing a contrast solution based on acetonitrile protection, preparing a sample solution by acetone ultrasonic treatment, GPC gel penetration chromatogram purification and cyclohexane-ethyl acetate elution, determining content of a plurality of pesticide residues in the pinellia ternate medicinal material by a gas chromatography-mass spectrometry combined method and carrying out concentration calculation. The method provided by the invention solves the technical problem of operation complexity and limitation of the traditional physicochemical property discriminating method, has high accuracy, can be operated simply, has a low cost and has a good application prospect and economic benefits. |
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