Higher-heat-stability amylase mutant, and preparation method and application thereof

Higher-heat-stability amylase mutant, and preparation method and application thereof

The invention discloses a higher-heat-stability amylase mutant, and a preparation method and application thereof, belonging to the field of enzyme engineering. A molecular biology technique is utilized to perform multiple site-directed mutagenesis to obtain the higher-heat-stability amylase mutant....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: DENG ZHUANGMEI, CHEN JIAN, YANG HAIQUAN, LIU LONG, DU GUOCHENG
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses a higher-heat-stability amylase mutant, and a preparation method and application thereof, belonging to the field of enzyme engineering. A molecular biology technique is utilized to perform multiple site-directed mutagenesis to obtain the higher-heat-stability amylase mutant. The half life of the obtained amylase at 60 DEG C is enhanced to 23.9 minutes from 3.2 minutes of the control sample (before mutation). The scheme can obviously enhance the heat stability of the amylase, and provides basis for industrial production. The scheme has important instruction meanings for property modification of other enzymes.