Human rotavirus vaccine and preparation method thereof

The invention discloses a human rotavirus vaccine. The human rotavirus vaccine is a combined vaccine which is formed by polyvalent pneumococcal polysaccharide and two or more than two of carrier proteins by virtue of connectors, wherein the connectors are magnetic nano-microspheres; one of the carrier proteins is a rotavirus protein. A preparation method of the human rotavirus vaccine comprises a step of respectively coupling the polyvalent pneumococcal polysaccharide and two or more than two of carrier proteins (one of the carrier proteins is the rotavirus protein) with the magnetic nano-microspheres. The human rotavirus vaccine is simple in preparation process; by adopting the human rotavirus vaccine using the nano-microspheres as the connector, the mice Th1-type immune response, and the immune persistence, specificity and affinity of the polysaccharide specific antibody can be increased; in addition, the mice can be induced so as to produce rotavirus antibodies; the human rotavirus vaccine has the prevention effect of two vaccines; therefore, the human rotavirus vaccine has very wide application prospect.