Method for accurately forecasting bacterial leaf blight of paddy rice at early stage by utilizing miRNA167c gene
The invention discloses a method for accurately forecasting bacterial leaf blight of paddy rice at early stage by utilizing miRNA167c gene, and belongs to the technical field of biology. The method comprises the following steps: selecting to-be tested paddy rice and control paddy rice, wherein the s...
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Zusammenfassung: | The invention discloses a method for accurately forecasting bacterial leaf blight of paddy rice at early stage by utilizing miRNA167c gene, and belongs to the technical field of biology. The method comprises the following steps: selecting to-be tested paddy rice and control paddy rice, wherein the selected control paddy rice is paddy rice which is not infected by bacterial leaf blight and is cultivated under same conditions with the to-be tested paddy rice; respectively separating total miRNA gene of the to-be tested paddy rice and the control paddy rice; respectively detecting the expression level of miRNA167c gene in the total miRNA gene of the to-be tested paddy rice and the control paddy rice, wherein the sequence of the miRNA167c gene is shown as SEQ ID NO: 1; and determining whether the experiment is successful according to the expression level of miRNA167c gene in the to-be tested paddy rice and the control paddy rice, if successful, further determining whether the to-be tested plant is infected by bac |
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