Preparation method of lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD

The invention provides a preparation and purification method of a lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD. The method comprises the steps as follows: seed amplification culture, fermented cultivation, centrifugal collection...

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Hauptverfasser: FAN YUMIN, CHEN GUANGMING, HUANG BIN, ZHANG HUANJING, RAO GUIRONG, MO GUOYU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a preparation and purification method of a lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD. The method comprises the steps as follows: seed amplification culture, fermented cultivation, centrifugal collection of thalli, thallus pyrolysis, supernatant collection and vacuum filtration, sieve chromatography, affinity chromatography, ion chromatography, isopropyl alcohol precipitation, PBS (phosphate buffer) dissolution and the like. With the adoption of the method, the fermentation content of the finally obtained lung cancer resistant plasmid T-VISA-BikDD is 150-200 mg/L, and the superhelix proportion of the plasmid after purification is higher than 90%.