Tide type bioreactor and method for culturing lily bulblets thereof
The invention innovatively utilizes a tide type bioreactor to rapidly propagate lily transplanting small bulblets. The bulblets are cultured in a conventional solid propagation culture medium to grow to obtain the lily small bulblets with the diameter of 0.2-0.4cm to be used as explants; the explant...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention innovatively utilizes a tide type bioreactor to rapidly propagate lily transplanting small bulblets. The bulblets are cultured in a conventional solid propagation culture medium to grow to obtain the lily small bulblets with the diameter of 0.2-0.4cm to be used as explants; the explants are inoculated into the tide type bioreactor; a liquid culture medium formula is replaced in a culturing process of 50 days; medicines are added to control the pollution and bulblet expansion and rooting are gradually carried out; after the bulblets are discharged out of a bottle, the lily bulblets are managed by conventional vernalization, transplanting and planting methods. According to the invention, bulblet expansion and rooting culture are carried out gradually and a tissue culture procedure can be simplified; the manual cost in conventional solid tissue culture is greatly reduced; the generation of pollution and variant strains can be effectively controlled; the lily breeding period is shortened and the pro |
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