Specific flavonoid probe substrate for sulfotransferase SULT1A3 and application thereof
The invention provides a specific flavonoid probe substrate for sulfotransferase SULT1A3 and application thereof. Determination of the activity of SULT1A3 comprises the following concrete operation procedures: selecting a flavonoid compound as a highly-specific probe substrate; establishing a reacti...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a specific flavonoid probe substrate for sulfotransferase SULT1A3 and application thereof. Determination of the activity of SULT1A3 comprises the following concrete operation procedures: selecting a flavonoid compound as a highly-specific probe substrate; establishing a reaction system used for research on the activity of SULT1A3, wherein the reaction system comprises SULT1A3, a substrate, a promoter, other auxiliary components, etc.; and quantitatively determining the elimination amount of the substrate or the production amount of a sulfated product thereof in unit time by regulating conditions consisting of the usage amount of SULT1A3, the concentration of the substrate, reaction temperature, the pH value of the system, reaction time and the like, thereby realizing investigation of the activity of SULT1A3 in each evaluation model. The high-efficiency and low-toxicity specific flavonoid probe substrate for sulfotransferase SULT1A3 provided in the invention can be used for quantitative |
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