Anti-Bel/fu monoclonal antibody and hybridoma cell line

The invention discloses a hybridoma cell line and an anti-Bel/fu monoclonal antibody secreted by the hybridoma cell line. The hybridoma cell line is obtained by fusing 5-fluorouracil human hepatocarcinoma cell line (Bel/fu) resistant immune mouse spleen cells and myeloma cells; the anti-Bel/fu monoc...

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Bibliographische Detailangaben
Hauptverfasser:	TIAN YANNA, SHE JINXIONG, XU HENG, DUN BOYING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a hybridoma cell line and an anti-Bel/fu monoclonal antibody secreted by the hybridoma cell line. The hybridoma cell line is obtained by fusing 5-fluorouracil human hepatocarcinoma cell line (Bel/fu) resistant immune mouse spleen cells and myeloma cells; the anti-Bel/fu monoclonal antibody has the functions of inhibiting the growth of Bel/fu, can be applied to diagnosis or treatment of tumors, and is contained in a preparation for diagnosing or treating the tumors. The invention also discloses a preparation method of the hybridoma cell line and the anti-Bel/fu monoclonal antibody. The anti-Bel/fu monoclonal antibody and hybridoma cell line disclosed by the invention can be used for preparing an anti-Bel/fu hybridoma cell line AC10364 by virtue of immune Bel/fu cells and secreting a corresponding monoclonal antibody AC10364, have effective activities for inhibiting the growth of various tumor cells, and can be applied to the diagnosis and treatment of liver cancers.